STR GENOTYPING

ACGT provides an amplicon size-based genotyping of Short Tandem Repeats (STRs) and microsatellites (MS). The application of fluorescent probe-tagged PCR primers and multiple ABI 3730XL Genetic Analyzers allows us to screen hundreds of samples each day. A high volume of samples can also be evaluated on the Fragment Analyzer™  Advanced Analytical Technologies (AAT) platform.

STR Genotyping Service Description:

• Design and synthesis of fluorescent-labeled PCR amplification primers for individual or multiplex strategies.
• Optimization of PCR amplification conditions for detecting STR and MS loci.
• PCR amplification of STR loci and separation on the ABI 3730XL or Fragment Analyzer.
• Allele size and identity determination with GeneMapper ID software.
• Verification of the data and allele calls by expert personnel.
• Data is presented in user-friendly spreadsheets that contain allele size and intensity.
• All results are available in electronic and hard copy formats including a publication quality electropherogram of each sample with a reference size ladder.
• Our personnel are experienced with difficult to assay samples and we provide assistance with data interpretation.
• A “load-and-go” service is available for customer-prepared fluorescently labeled PCR products.