SAMPLE SUBMISSION GUIDELINES
Samples can be submitted in two different formats: raw material (tissue, cells, soil, etc.), or purified nucleic acids (DNA or RNA). Please read carefully the description of the quantity and quality requirements for each type of sample.
A failure to provide the required amounts and quality of sample material may downgrade the performance of service(s) and/or make the completion of the order unattainable.
DNA/RNA Extraction Services
Details of Service:
1. All extracted DNA samples will be evaluated for quantity and quality with NanoDrop spectroscopy and/or Qubit fluorometry and/or agarose gel electrophoresis, as appropriate.
2. All extracted RNA samples will be evaluated for quantity and quality (RIN values) with NanoDrop spectroscopy and/or Qubit fluorometry, and 2100 Bioanalzyer with RNA 6000 chip.
3. Any significant issues with sample quantity or quality shall be communicated to the individual requesting the service.
| DNA/RNA Extraction Services | Amount of Required Material* |
| DNA or RNA extraction -standard (blood, soft tissue, swabs, saliva, cells, bacteria and yeasts) | 0.5 mL of blood; 20 mg of tissue, 2 swabs, 1 mL of saliva, 106 cells, 109 cells or several colonies on a plate |
| DNA extraction -difficult (FFPE, bone, biofilm, soil, fungi spores, plants) | 5 slides, 100 mg of tissue, 50 mg of biofilm, 1 gm of soil, 100 mg of fungal material, 200 mg of plant tissue |
| RNA extraction – standard | 0.5 mL of blood; 20 mg of tissue, 2 swabs, 1 mL of saliva, 106 cells |
| RNA extraction – difficult | 100 mg of tissue; 50 mg of biofilm, 1 gm of soil, 100 mg of fungal material, 200 mg of plant tissue |
| RNA extraction FFPE | 6 slides or equivalent |
*The amounts identified above are the minimum requirements. We strongly recommend that your submission exceed these standards, if possible.
DNA / RNA samples
| Type of Material | Amount of Material | Sample Quality Requirements |
| Genomic DNA | 100 ng | OD260/280 between 1.6 and 2.0;OD260/230 more than 1.6; concentration between 20 and 500 ng / µL |
| DNA amplicons | 50 ng/µl per band as determined by 2100 Bioanalyzer or Fragment Analyzer | |
| Total RNA | 2,000 ng | OD260/280 more than 2.0;OD260/230 more than 1.8; concentration between 20 and 500 ng / µL; RIN>8.0 |
| mRNA | 500 ng |