SAMPLE SUBMISSION GUIDELINES
Samples can be submitted in two different formats: raw material (tissue, cells, soil, etc.), or purified nucleic acids (DNA or RNA). Please read carefully the description of the quantity and quality requirements for each type of sample.
A failure to provide the required amounts and quality of sample material may downgrade the performance of service(s) and/or make the completion of the order unattainable.
DNA/RNA Extraction Services
Details of Service:
1. All extracted DNA samples will be evaluated for quantity and quality with NanoDrop spectroscopy and/or Qubit fluorometry and/or agarose gel electrophoresis, as appropriate.
2. All extracted RNA samples will be evaluated for quantity and quality (RIN values) with NanoDrop spectroscopy and/or Qubit fluorometry, and 2100 Bioanalzyer with RNA 6000 chip.
3. Any significant issues with sample quantity or quality shall be communicated to the individual requesting the service.
|DNA/RNA Extraction Services||Amount of Required Material*|
|DNA or RNA extraction -standard (blood, soft tissue, swabs, saliva, cells, bacteria and yeasts)||0.5 mL of blood; 20 mg of tissue, 2 swabs, 1 mL of saliva, 106 cells, 109 cells or several colonies on a plate|
|DNA extraction -difficult (FFPE, bone, biofilm, soil, fungi spores, plants)||5 slides, 100 mg of tissue, 50 mg of biofilm, 1 gm of soil, 100 mg of fungal material, 200 mg of plant tissue|
|RNA extraction – standard||0.5 mL of blood; 20 mg of tissue, 2 swabs, 1 mL of saliva, 106 cells|
|RNA extraction – difficult||100 mg of tissue; 50 mg of biofilm, 1 gm of soil, 100 mg of fungal material, 200 mg of plant tissue|
|RNA extraction FFPE||6 slides or equivalent|
*The amounts identified above are the minimum requirements. We strongly recommend that your submission exceed these standards, if possible.
DNA / RNA samples
|Type of Material||Amount of Material||Sample Quality Requirements|
|Genomic DNA||100 ng||OD260/280 between 1.6 and 2.0;OD260/230 more than 1.6; concentration between 20 and 500 ng / µL|
|DNA amplicons||50 ng/µl per band as determined by 2100 Bioanalyzer or Fragment Analyzer|
|Total RNA||2,000 ng||OD260/280 more than 2.0;OD260/230 more than 1.8; concentration between 20 and 500 ng / µL; RIN>8.0|