logo
fragment analysis header

STR Genotyping print this page   email this page


fragment analysis graphACGT, Inc. offers a complete Fragment Analysis of Short Tandem Repeats (STR) or microsatellite (MS) genotyping service. Use of multiple ABI 3730XL or 3730 Genetic Analyzers allows us to screen hundreds of samples per day. STR Genotyping is available for most organisms.

STR Genotyping Service Description:

  • Design and synthesis of fluorescent-labeled PCR amplification primers for individual or multiplex strategies.
  • Optimization of PCR amplification conditions for detecting STR and MS loci.
  • PCR amplification of STR loci and separation on the ABI 3730XL or 3730 Genetic Analyzer.
  • Allele size and identity determination with GeneMapper ID (V3.0) software and experimenter
  • verification of the data.
  • Data presented in user-friendly spreadsheets that contain allele size and intensity.
  • All results are available in electronic and hard copy formats including a publication quality electropherogram of each sample with a reference size ladder.
  • Experience with difficult to assay samples and complete assistance with data interpretation.
  • A "load-and-go" service is available if the user has prepared the fluorescently labeled PCR products and only requires analysis of these on the Genetic Analyzer.