Shotgun DNA Sequencing

ACGT, Inc. offers specialty-sequencing services for large plasmids, BAC, PAC, cosmid DNA using shotgun DNA sequencing method.

Library Construction and Sequencing

ACGT, Inc. provides shotgun sequencing services for a variety of samples including BAC, PAC, or cosmid DNA for genomic scale projects.

Shotgun DNA Sequencing Service Description :

• Shotgun library construction by shearing of the DNA sources
• Size selection of fragmented DNA
• Subsequent subcloning of fragments into a cloning vector
• Library quality control check. E-coligenomic DNA contamination and empty vector contamination by cross-match. Less than 5% contamination will be accepted
• Plasmid purification of selected clones
• Primer extensions with forward and reverse primers
• Assembly of the overlapping sequences
• Completion for 100% coverage of the DNA by primer walking
• Determination of the consensus sequence

You will receive:

• Assembled and based-called DNA sequences
• Publication-ready DNA sequences
• Electropherograms of each run
• All of the subclones, plasmid DNA, and internal primers generated for the project