Sample Submission Guidelines    

Samples can be submitted in three different formats: raw materials (tissue, cells, soil, purified nucleic acids, or prepared libraries¹.

General sequencing (genomic DNA, BACs, YACs, cosmids, PCR amplicons, etc.)

Submit at least 100 mg tissue for DNA extraction. If submitting non-tissue samples, please provide enough material to extract at least 2 µg of purified DNA. If submitting DNA, send ≥ 2 µg of purified DNA in 50 µl TE buffer. Sample quality and quantity will be verified by fluorometry (pico green assay), spectrophotometry (NanoDrop 1000) and densitometry (1-2% agarose gel with DNA mass ladder).

mRNA-seq²

Submit at least 100 mg of relevant tissue or cells, or enough non-tissue material to extract at least 0.4 µg total RNA. If submitting purified RNA, submit 0.4-1 µg DNAse treated total RNA (or mRNA, purified from 0.4-1 µg total RNA), in 10 µl ultra pure water. For prokaryote RNA analysis, it is strongly recommended that the total RNA be depleted of rRNA (we recommend various RiboZero kits from Epicenter). Actually, it is a good idea for eukaryote RNA as well.

Small RNA²

Submit at least 100 mg of relevant tissue or cells, or enough non-tissue material to extract at least 5 µg total RNA. If submitting purified RNA, submit 5 µg total RNA (or small RNA extracted from 5 µg total RNA) in 10 µl ultra pure water.

ChIP-Seq and Target Capture

Submit 100-500 ng ChIP or capture kit enriched DNA in 30 µl ultra pure water. Sample must be accompanied by qPCR verification.

¹Clients preparing their own libraries should submit an aliquot of the stock library; we will evaluate it and dilute it to an appropriate concentration.

²All RNA samples must be accompanied by an Agilent 2100 Bioanalyzer profile and must have an RIN (RNA integrity number) of 7 or higher. Samples with lower RIN numbers could potentially generate sequences with 3' bias.