Sample Submission
Please see below for details on how samples should be submitted to ACGT, Inc.
IN A SEPERATE TUBE FOR TEMPLATE AND PRIMER:
| Template DNA | Size of DNA | Amount (ng) | Primer (pmol) |
| Plasmid DNA | up to 10 kb | 100 | 10 - 15 |
| PCR DNA | 100 - 200 bp | 5 | 10 - 15 |
| PCR DNA | 200 - 500 bp | 10 | 10 - 15 |
| PCR DNA | 500 bp - 1 kb | 20 | 10 - 15 |
| PCR DNA | 1 kb - 2 kb | 50 | 10 - 15 |
| PCR DNA | over 2 kb | 100 | 10 - 15 |
| Large DNA | over 10 Kb | 500 - 1,000 | 20 |
*Please send enough DNA to do a re-run in case the first reaction fails.
PRE-MIXED INSTEAD OF SENDING IN SEPARATE TUBE:
| Template DNA | Size of DNA | Amount (ng) | Total Vol. | Primer (pmol) |
| Plasmid DNA | up to 10kb | 100 - 200 | 10 µl | 20 |
| PCR DNA | 100 - 200bp | 5 - 10 | 10 µl | 20 |
| PCR DNA | 200-500bp | 10 - 20 | 10 µl | 20 |
| PCR DNA | 500 bp - 1kb | 20 - 30 | 10 µl | 20 |
| PCR DNA | 1 kb - 2kb | 50 - 100 | 10 µl | 20 |
| PCR DNA | over 2kb | 100 - 150 | 10 µl | 20 |
| Large DNA | over 10Kb | 500 - 1,000 | 20 µl | 20 - 40 |
*Note that if a template needs to be analyzed in two directions, pre-mixed samples of the template
with both forward and reverse primers should be submitted as separate reactions.
LIGATION MIXTURE FOR TRANSFORMATION
Ligation mixtures are processed by electroporation
to transform into suitable host cells. They are further
propagated for sequencing or other molecular biology
procedure.
Ligation mixtures should be ethanol precipitated, frozen
in water, and shipped in screw top tubes in a frozen
(dry ice) package.
TRANSFORMATION SAMPLES FOR PLATING AND PICKING
- Transformation samples should be frozen and shipped
in screw top tubes in a dry ice package.
- Suitable antibiotics and their concentration for clone
selection:
| Antibiotic | Final Concentration |
| Chloramphenicol | 12.5 g / ml |
| Carbenicillin/Ampicillin | 50 g / ml |
| Kanamycin | 35 g / ml |
| Zeocin* | 25 g / ml |
- Templates which contain Zeocin-resistant gene should
be grown in low salt LB media.
- Submit enough vials of frozen, never thawed, material
each time, since the titer usually drops after freezing and
thawing.
- Submit at least twice the number of required colonies for
each sample and provide titer information
on the order
form.
SAMPLE SUBMISSION GUIDELINES
Please see below for details how samples should be submitted to ACGT, Inc.
DNA Sample Submission for Quick Single Pass Sequencing
DNA Sample Submission for Other Services
DNA Sample Submission for High-throughput Sequencing
Sample Submission for Molecular Biology Project
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