Sample Shipping of DNA sequencing

Sample Shipping

Please follow the instructions below to learn how to send your samples in the best possible way in order to complete your order.

dna sequencing sample shipping IN GENERAL

- ACGT, Inc. accepts samples Monday through Friday from all carriers and on Saturday from Fed-Ex.
  To ensure your samples are received on Saturday, please mark “Saturday Delivery” on the Fed-Ex
  Airbill.

- The order form or a detailed description of the submitted samples must be enclosed in the package.
  Processing of any shipment without proper paperwork will be delayed.

- Please send samples to: ACGT, Inc.
                                      35 Waltz Drive
                                      Wheeling, IL 60090

- DNA samples in tubes may be shipped at room temperature for overnight delivery. It is not
  necessary to send the samples on dry ice or wet ice.

- Template DNA stored in individual tubes should be capped tight to prevent accidental spillage or
  cross contamination.

- DNA shipped as a pellet or in EtOH can be also be shipped at room temperature.

- For Same-Day Service, DNA Sequencing samples must be sent by Fed-Ex First Overnight. Please
  view the Fed-Ex Airbill carefully.

- Please ship the DNA samples with adequate padding to prevent any damage during shipment. We
  provide bubble bags for your shipments. Please request bubble bags if you need them for your
  future orders.

dna sequencing sample shipping DNA SEQUENCING SAMPLES IN 96-WELL PLATE

- It is necessary to fr eeze the samples and ship on dry ice. Pack the plates, while still frozen on dry
  ice, in a styrofoam box. Make sure there is enough dry ice on the top, bottom, and both sides of the
  plates.

- Plates should be sealed properly with a polystyrene sealer to avoid accidental spillage or cross
  contamination.

- If shipping more than one plate, wrap the plates in aluminum foil and/or vinyl tape (not lab tape) so
  they will not move around during shipment. Lab tape will become brittle and rip when exposed to   dry ice.

dna sequencing sample shipping BACTERIAL AGAR PLATES

- Wrap the edges of the plates with parafilm and place them in the container, with the lid side down.
  Pack with protective bubble pad.

- Plates can be shipped at room temperature if not overgrown.

dna sequencing sample shipping BACTERIAL AGAR LAWNS

- Wrap the edges of the lawns in parafilm.

- Pack agar lawns in a styrofoam cooler. Plates should be cooled to room temperature to prevent
  condensation. Once the plates have cooled, place them in the cooler with the lid side down.

- Place ice packs in the cooler. Do not place ice packs directly on plates as condensation may leak
  into the plates. Make sure there are “THIS END UP” arrows on the box.

Sample Shipping Guideline For Molecular Biology Project

dna sequencing sample shipping TISSUE OR CELLS FOR cDNA OR GENOMICLIBRARY CONSTRUCTION

- Sponsor should submit at least 1g of tissue or 1x10 zcells for a cDNA library construction.

- In order to obtain RNA of high quality and yield, fresh or instantly frozen tissues or cell cultures are   required.

- All tissues or cells need to be quickly prepared and flash frozen in liquid nitrogen to minimize RNA   degradation. These samples should be stored at –80°C until used.

- Information on Sample ID, sample collection documentation, and packaging information should be   submitted along with samples. Upon receipt, the integrity of sample containers will be checked by   analysts and relevant information will be recorded on the sample log-in record or a laboratory   notebook.

- Samples should be shipped frozen on dry ice by overnight delivery, packed with protective bubble   pad. Make sure dry ice surrounds all sides of the samples.

cDNA OR GENOMIC LIBRARY

- Samples with DMSO should be shipped frozen by overnight delivery, packed with protective bubble   pad. Make sure dry ice surrounds all sides of the specimens.

- Samples without DMSO should be shipped refrigerated or with a wet/blue ice package, packed with   protective bubble pad.

WHOLE BLOOD OR BUFFY COAT SAMPLES

- Samples should be collected in suitable collection media such as QIAGEN PAXGene solution, stored   refrigerated (fresh, never frozen) or frozen, maintained at proper temperature (4°C, -20°C, or
  -80°C) and submitted in a refrigerated or frozen package.

- Fresh samples should be shipped refrigerated, with a wet/blue ice package, packed with protective   bubble pad.

- Frozen samples should be shipped frozen by overnight delivery, packed with protective bubble pad.   Make sure dry ice surrounds all sides of the specimens.

TOTAL RNA OR mRNA FOR cDNA LIBRARY CONSTRUCTION

- Total RNA or mRNA should be isolated from tissue or cells using commercial kits or equivalent   methods, resuspended in nuclease free water, stored at -80°C and submitted in a frozen package.

- Sponsor should submit at least 1mg of total RNA or 5µg of mRNA for construction of a standard   cDNA library.

- Sponsor should submit A260/A280 readings and gel pictures along with the samples. A260/A280   should be at least 1.8. The upper ribosomal RNA band should be about twice the intensity of the   lower ribosomal RNA band.

- Samples should be shipped frozen on dry ice by overnight delivery.