NEXT GENERATION SEQUENCING

ACGT is committed to using the most proven and up-to-date technologies in our Next Generation Sequencing (NGS) service. We provide SBS (sequencing by synthesis) TruSeq technology from Illumina®/Solexa, the most successful NGS platform adopted worldwide. TruSeq’s dependable base-by-base sequencing delivers the industry’s most accurate data for a broad range of applications. Furthermore, ACGT uses only data that has met or exceed the Q30 (i.e. 1 out of 1,000 chance of error) threshold in our analyses.

Platforms and Capabilities

Our Illumina MiSeq®, HiSeq4000® and NextSeq500® instruments are established and versatile platforms for genomic discovery and validation.

Illumina MiSeq®
Capacity: Single lane flow cell
Maximum Clusters: 20 to 25 million (up to 50 million paired-end reads)
*Maximum Run: 2 x 300 bp paired-end, 65 hours, up to 12 Gb of sequence data
Expected PE300 Read Quality: > 70% Q30 bases per run
Illumina NextSeq500®
Capacity: Mid Output Mode Run High-Output Run
*Maximum Clusters: 120 million PE reads 400 million PE reads
*Maximum Run: 2 x 150 bp paired-end, 26 hours, up to 36 Gb of sequence data 2 x 150 bp paired-end, 29 hours, up to 110 Gb of sequence data
Expected PE150 Read Quality: > 75% Q30 bases per run > 75% Q30 bases per run
Illumina HiSeq4000®
Capacity: One 8-lane flow cell Two 8-lane flow cells
*Maximum Clusters: 2.5 billion PE reads 5 billion PE reads
*Maximum Run: 2 x 150 bp paired-end, 3.5 days, up to 750 Gb of sequence data 2 x 150 bp paired-end, 3.5 days, up to 1,500 Gb of sequence data
Expected PE150 Read Quality: > 75% Q30 bases per run > 75% Q30 bases per run

# While a total of 384 samples can be uniquely barcoded with Nextera Illumina indexes, 1 barcode may be reserved for the PhiX control on all lanes.

*Based on the v3 Illumina kits

Next Generation Sequencing Applications We Support

The following are included with our NGS services:

  • Extensive project consultation to help you optimize your experiment
  • Complete Quality Control analysis during library preparation
  • Onsite sample processing and custom library preparation
  • Onsite RT-qPCR analysis available for evaluation of key transcript levels in sample RNA
  • Data processing and bioinformatics analysis can be performed entirely onsite if requested, for maximum data security and confidentiality
  • High quality raw sequence data (provided as FASTA files) and all bioinformatics analysis files are shipped on a drive or provided through a secure FTP link
  • Multiple data analysis options are available
  • Full project report
  • Sanger sequencing is available onsite for gap closure, SNP validation and junction sequence confirmation

NGS Service FAQs

Dr. Semyon Rubinchik, Scientific Director, answers frequently asked questions about our NGS services.

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