NEXT GENERATION SEQUENCING

ACGT is committed to using the most current and proven technologies in our Next Generation Sequencing (NGS) service. We apply SBS (sequencing by synthesis) TruSeq technology from Illumina®/Solexa, the most successful NGS platform adopted worldwide. TruSeq’s dependable base-by-base sequencing delivers the industry’s most accurate data for a broad range of applications. Furthermore, ACGT uses only data that has passed the Q30 (i.e. 1 out of 1,000 chance of error) filter in our analyses.

For a listing of our prices for NGS services, please see our Price Schedule.(PDF)

Platforms and Capabilities

Our Illumina MiSeq®, HiSeq2500® and NextSeq500® instruments are established and versatile platforms for genomic discovery and validation.

Illumina MiSeq®
Capacity: Single lane flow cell
Maximum Clusters: 20 to 25 million (up to 50 million paired-end reads)
*Maximum Run: 2 x 300 bp paired-end, 65 hours, up to 12 Gb of sequence data
Expected PE300 Read Quality: > 70% Q30 bases per run
Illumina NextSeq500®
Capacity: Mid Output Mode Run High-Output Run
*Maximum Clusters: 120 million PE reads 400 million PE reads
*Maximum Run: 2 x 150 bp paired-end, 26 hours, up to 36 Gb of sequence data 2 x 150 bp paired-end, 29 hours, up to 110 Gb of sequence data
Expected PE150 Read Quality: > 75% Q30 bases per run > 75% Q30 bases per run
Illumina HiSeq4000®
Capacity: One 8-lane flow cell Two 8-lane flow cells
*Maximum Clusters: 2.5 billion PE reads 5 billion PE reads
*Maximum Run: 2 x 150 bp paired-end, 3.5 days, up to 750 Gb of sequence data 2 x 150 bp paired-end, 3.5 days, up to 1,500 Gb of sequence data
Expected PE150 Read Quality: > 75% Q30 bases per run > 75% Q30 bases per run

# While a total of 384 samples can be uniquely barcoded with Nextera Illumina indexes, 1 barcode may be reserved for the PhiX control on all lanes.

*Based on the v3 Illumina kits

Next Generation Sequencing Applications We Support

The following are included with our NGS services:

  • Extensive project consultation to help you optimize your experiment
  • Complete Quality Control analysis during library preparation
  • Onsite sample processing and custom library preparation
  • Onsite RT-qPCR analysis available for evaluation of key transcript levels in sample RNA
  • Data processing and bioinformatics analysis can be performed entirely onsite if requested, for maximum data security and confidentiality
  • High quality raw sequence data (provided as FASTA files) and all bioinformatics analysis files are shipped on a drive or provided through a secure FTP link
  • Multiple data analysis options are available
  • Full project report
  • Sanger sequencing is available onsite for gap closure, SNP validation and junction sequence confirmation

NGS Service FAQs

Dr. Semyon Rubinchik, Scientific Director, answers frequently asked questions about our NGS services.

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