LARGE SCALE SNP ANALYSIS
For the analysis of hundreds of targets in a large volume of samples, ACGT offers genotyping by the KASP Assay. The KASP genotyping chemistry is based on a competitive allele-specific PCR and it enables bi-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (indels) at specific loci.
Multiple 384 or 1536-well plates are loaded onto thermocyclers. Upon the completion of PCR, all genotyping reaction plates are read on a BMG PHERAStar plate reader. Software by Kraken calls genotypes automatically. Thereafter, a project manager will access the plate read data and apply a detailed, thorough data analysis. This may require correction of the automatically called genotypes. To ensure data quality, a second team specialist will re-check these results to verify or apply any changes, if needed.
ACGT also offers Genotyping By Sequencing (GBS) on Illumina NGS platforms. The industry-leading technology of Illumina® yields a high sample throughput with high quality data required for accurate SNP discovery and structural variation analysis studies. This approach is well suited for the discovery of novel SNPs in plant and animal genomes as well as genotype-based population and individual identification of all species.
All results are available in electronic and hard copy formats. Assistance with data interpretation can be provided upon request.