Quick Single Pass DNA Sequencing

ACGT, Inc. offers Quick Single Pass DNA Sequencing service for plasmid, PCR DNA,
and large-insert templates (lambda, BAC, PAC, P1 clones).
DNA Sequencing Service Description:
- DNA sequencing reactions using BigDye terminator version 3.1
- Clean-up using CleanSEQ dye terminator removal kit
- Analysis of the extension products using ABI 3730XL or 3730 Genetic Analyzer
- Data review and editing for every sample
- Extensive trouble shooting for problematic samples
- Customer support 24/7
- Repeat Policy: If the first reaction does not produce usable data,
the reaction will be
repeated under modified reaction conditions at no extra charge.
TURNAROUND TIME
| Service | Turnaround Time | |
| Same Day | Data return by 6:00pm CST on the same day DNA is received by ACGT, Inc. | |
| Standard | Next day by noon. | |
SAME
DAY DATA RETURN SERVICE
ACGT, Inc. offers a Same Day Data Return Service to meet your urgent data request. By utilizing this service, you will receive your DNA sequencing results by 6:00pm CST on the same day that we receive your DNA Sequencing samples during the weekdays. We also offer Same Day Service on Saturday. The data for the Saturday samples will be returned by Monday morning.
Ship the DNA samples via Fed-Ex First Overnight delivery. We accept only Fed-Ex shipment for Same Day Data Return Service. Please click here to view Fed-Ex packing slip.
QUALITY
We generate more than 1,000 bases of clean and edited data for plasmid and PCR DNA. View a typical analysis of pGEM control plasmid DNA. Our first pass rate is 96-98% with customer plasmid DNA samples, 92-96% with customer PCR DNA samples, and 98-99% with pGEM control plasmid DNA. With this high success rate, you are likely to receive all the data the very next day after sending your samples to us.
UNIVERSAL PRIMERS
ACGT, Inc. provides 50 different universal primers at no additional charge.
View Primer List.
PRIMER BANKING
For your convenience upon your request, we bank your specific internal primers for future use at no extra charge. You can also view your stored primer list in your personalized web page.
View Example.
HARD TO SEQUENCE SAMPLES
We have extensive experience with high GC DNA, AT rich DNA, DNA with secondary structures, DNA with repetitive sequences, and dirty DNA, plasmid cloned in Gateway vector, etc. Trouble-shooting or problem solving support is available from our Ph.D. level scientists any time of the day.
"LOAD & GO" DNA SEQUENCING SAMPLES:
With this service, you perform your own DNA sequencing reactions and send us the cleaned
samples for sequencing. Samples can be submitted either in liquid or dried format.
DNA Sequencing Service Description:
- Analysis of your reactions on an ABI 3730XL or 3730.
- A thorough examination of the chromatograms and data editing.
- Data review of each sample and return of review summary.
- Next day data return for standard service.
-
"Same Day Service" available.
PLASMID DNA PREPARATION FROM BACTERIAL CLONES
We purify your DNA in a single tube or 96-well plate for your
convenience and perform DNA sequencing.
GEL OR COLUMN PURIFICATION OF PCR PRODUCTS
For your unpurified PCR product, we will column purify it prior to sequencing if your PCR
amplification product contains a single robust band. If it contains multiple bands, gel
purification of the band with the desired size will be performed.
OLIGO DESIGN AND SYNTHESIS
If your project requires specific internal primers, ACGT, Inc. offers Oligo Design and
Synthesis service on a small scale for your convenience.
- Based on previously generated sequence data, oligo synthesis and additional primer
extension
reactions can be performed within two days.
- Return of oligos or long-term storage for future projects.
FREE DNA SEQUENCING SAMPLE SHIPPING
You can drop off your DNA Sequencing samples at any of our Drop-Boxes if your facility has one. Our local courier consolidates all the packages and ships them to us to our Chicago location by overnight delivery via FedEx using our account.
OPEN ON SATURDAYS
We are open on Saturdays so you can send your DNA Sequencing samples on Friday and have your data by Monday morning instead of waiting until Monday to send out your DNA Sequencing samples.


