qPCR    

ACGT, Inc. offers a rapid, reliable semi-quantitative and quantitative PCR (qPCR) analysis of gene expression and gene copy number determination. Detection of targets with Taqman™ probes with the 7900HT Sequence Detection System (Applied Biosystems Inc.) or SYBR green is available for large or small scale projects alike. The instrument platform has high-throughput capabilities and can analyze over 1,500 samples on a daily basis.

The full service option includes RNA or DNA extraction, probe design and optimization and complete data analysis including normalization of samples with internal controls. Relative and absolute analysis of gene expression by qPCR is available for all known genes in any organism.

Service Description:

• Extraction of RNA or DNA from a variety of samples
• Design of customized PCR primers and probe.
• Optimization of PCR amplification conditions for target detection
• Development and generation of cDNA or DNA standard curves for relative or absolute quantification
• Determination of the relative or absolute transcript or gene copy number
• Data presented in user-friendly spreadsheets that contain raw and normalized Ct values
• All results are available in electronic and hard copy formats including a publication quality fluorescence graph of each sample and the standard curve.
• Experience with difficult to assay samples and complete assistance with data interpretation