Next Generation Sequencing    

Massively Parallel Sequencing technologies offer powerful techniques for unlimited discovery in all areas of genetic and genomic research. With these tools, you can gain unexpected insights, find unprecedented answers, and publish your results faster than ever.

Technology we use

At ACGT, Inc., we are using state of the art SBS (sequencing by synthesis) TruSeq technology from Illumina / Solexa. This is the most successful and widely-adopted next-generation sequencing platform worldwide.

TruSeq technology employs a proprietary reversible terminator-based method that enables detection of single bases as they are incorporated into growing DNA strands. After each cycle, each tile of the flow cell is imaged for each fluorescently-labeled terminator nucleotide. All images are analyzed and base calls are made for each cycle. Since all four reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias.

The end result is true base-by-base sequencing that delivers the industry’s most accurate data for a broad range of applications.

ACGT, Inc.’s Illumina Genome Analyzer IIx offers a powerful combination of 2 x 150 bp read lengths and up to 640 million paired-end reads per flow cell. This translates into a maximum of 80 Gb of DNA sequence per run. With Illumina barcode system, up to 96 different samples can be analyzed per run enabling a broad range of high-throughput sequencing applications.

Illumina sequencing technology with the Genome Analyzer IIx is a proven platform for genomic discovery and validation.

Applications we support

• Whole and Targeted Genome Sequencing Analysis »
• Transcriptome (RNA-Seq) and small RNA Analysis »
• DNA Methylation Analysis »
• ChIP-Seq Analysis »
• Metagenomic Analysis »

The ACGT, Inc. Advantage

• Extensive project consultation to help you optimize your experiment
• Complete Quality Control analysis during library preparation
• Onsite sample processing and custom library preparation
• Onsite RT-qPCR analysis is available for evaluation of key transcript levels in sample RNA
• Data processing and bioinformatics analysis are performed entirely onsite, for maximum data security and confidentiality
• High quality raw sequence data (provided as FASTA files) as well as all bioinformatics analysis files are shipped to the customer on a secured hard drive.
• Multiple data analysis options are available
• Full project report
• Sanger sequencing is available onsite for gap closure, SNP validation and junction sequence confirmation
• Advantageous pricing options are available for multiplexed samples