High-Throughput Dna Sequencing

High Throughput DNA Sequencing

ACGT, Inc. offers High throughput DNA sequencing in a fully automated lab. We are capable of handling thousands of clones per day using a barcode system to accurately inventory and process DNA samples upon receipt until delivery of final data. Project tracking for each stage of sample processing can be viewed realtime online via our website.

High Throughput DNA Sequencing Service Description:

  • DNA template preparation by column purification
  • Checking template and primer DNA using Nanodrop or gel electrophoresis
  • Cycle sequencing reactions using BigDye terminator version 3.1 from ABI
  • Clean-up using CleanSEQ dye terminator removal kit on High Throughput Dna Sequencing Biomek Automation Station from Beckman Coulter, Inc.
  • Analysis of the extension products using ABI 3730XL or 3730 Genetic Analyzer
  • A thorough examination of the chromatograms and data editing
  • Data review of each sample and return of review summary
  • Return of 600-900 bases of edited and vector clipped sequences to optimize the database searches
  • An initial assessment of the library with one plate of 96 clones upon request
  • BLAST search upon request
  • Universal primers at no additional charge
  • Return of plasmid DNA sequencing samples subsequently catalogued

Related Services

"Load & Go" Service:

With this service, you perform your own DNA sequencing reactions and send us the cleaned
samples for sequencing. Samples can be submitted either in liquid or dried format.

High Throughput DNA Sequencing Service Description Service Description:

  • Analysis of your reactions on an ABI 3730XL or 3730
  • A thorough examination of the chromatograms and data editing
  • Data review of each DNA sequencing sample and return of review summary
  • Next-day data return for standard service
  • "Same Day Service" available
  • Full-line support such as repeated runs, trouble shooting, and technical support at no extra charge

Plasmid DNA preparation from bacterial clones:
We purify your DNA in a 96-well plate, or 384-well plates for your convenience and perform DNA

Column purification of PCR products:
For your unpurified PCR product, we column purify DNA in a 96-well plate prior to DNA sequencing.