Guidelines for Sample Submission and Shipping for Molecular Biology Project    

Please review the details below for how Molecular Biology Project test acticles should be submitted and shipped to
ACGT, Inc.

Tissue or Cells for cDNA or Genomic Library    Construction - Sponsor should submit at least 1g of tissue or   1x10 zcells for a cDNA library construction. - In order to obtain RNA of high quality and yield,   fresh or instantly frozen tissues or cell cultures are   required. - All tissues or cells need to be quickly prepared and   flash frozen in liquid nitrogen to minimize RNA   degradation. These samples should be stored at   –80°C until used. - Information on Sample ID, sample collection   documentation, and packaging information should be   submitted along with samples. Upon receipt, the   integrity of sample containers will be checked by   analysts and relevant information will be recorded on   the sample log-in record or a laboratory notebook. - Samples should be shipped frozen on dry ice by   overnight delivery, packed with protective bubble   pad. Make sure dry ice surrounds all sides of the   samples.  cDNA or Genomic Library - Samples with DMSO should be shipped frozen by   overnight delivery, packed with protective bubble   pad. Make sure dry ice surrounds all sides of the   specimens. - Samples without DMSO should be shipped   refrigerated or with a wet/blue ice package, packed   with protective bubble pad.

Whole Blood or Buffy Coat Samples - Samples should be collected in suitable collection   media such as QIAGEN PAXGene solution, stored   refrigerated (fresh, never frozen) or frozen,   maintained at proper temperature (4°C, -20°C, or   -80°C) and submitted in a refrigerated or frozen   package. - Fresh samples should be shipped refrigerated, with a   wet/blue ice package, packed with protective bubble   pad. - Frozen samples should be shipped frozen by   overnight delivery, packed with protective bubble   pad. Make sure dry ice surrounds all sides of the   specimens.   Total RNA or mRNA for cDNA Library    Construction - Total RNA or mRNA should be isolated from tissue or   cells using commercial kits or equivalent methods,   resuspended in nuclease free water, stored at -80°C   and submitted in a frozen package. - Sponsor should submit at least 1mg of total RNA or   5µg of mRNA for construction of a standard cDNA   library. - Sponsor should submit A260/A280 readings and gel   pictures along with the samples. A260/A280 should   be at least 1.8. The upper ribosomal RNA band   should be about twice the intensity of the lower   ribosomal RNA band. - Samples should be shipped frozen on dry ice by   overnight delivery.