Genotyping by PCR    

ACGT, Inc. offers a complete Genotyping by PCR service for screening alleles based on gene structure. Alleles in any organism are detected by identifying unique nucleotide elements in the target gene(s) of genomic DNA (or RNA via cDNA) at the PCR amplification stage, in the PCR product or both. This strategy is an effective and efficient method for detecting and screening gene insertions, deletions, or rearrangements in natural or artificial gene constructs.

 Genotyping by PCR Service   Description:

- Complete study design and support   capabilities
- Design and synthesis of PCR amplification   primers
- Optimization and verification of PCR   amplification conditions for new and pre-existing strategies
- Isolation of genomic DNA, RNA and cDNA synthesis
- PCR amplification, post PCR restriction digests (if needed) and gel analysis
- Sample genotyping and verification by at least two experts
- Sample verification with repeated analysis
- Extensive troubleshooting experience and technical support
- Data presented in user-friendly spreadsheets with genotypes and in publication quality photos
  of gel analysis
- Extensive troubleshooting experience and technical support
- FDA quality DNA sequence data available
- Complete GLP capabilities