Genotyping by DNA Sequencing    

ACGT, Inc. offers a complete Genotyping by DNA Sequencing for screening alleles based on nucleotide sequence. Alleles in any organism are detected by PCR amplification of the target gene and DNA sequencing of the resulting PCR product. Dilution PCR analysis or bulk PCR product subcloning and screening followed by DNA sequencing is an additional service provided for reliable analysis of single point mutations in low abundance. DNA sequencing is one of the most definitive methods available for allele identification and genotyping.

 Genotyping by DNA Sequencing
  Service Description:

- Complete study design and support   capabilities
- Design and synthesis of PCR amplification   primers
- Optimization and verification of PCR amplification conditions for new and pre-existing strategies
- Isolation of genomic DNA, RNA and cDNA synthesis
- PCR amplification and DNA sequencing
- Dilution PCR analysis or bulk PCR product subcloning/screening followed by DNA sequencing
- Manual verification of automated base calls in sequence data
- Phred 20-40 analysis of sequence data
- Sequence data comparison analysis between samples and reference sequence
- Extensive troubleshooting experience and technical support
- FDA quality DNA sequence data available
- Complete GLP capabilities