Gene Mutagenesis    

ACGT, Inc. offers a complete mutagenesis service for creating single or multiple point mutations, deletions, insertions or random mutations into any target gene. Fast and efficient mutagenesis methods allow us to quickly create novel constructs for large and small projects alike.


 SITE-DIRECTED MUTAGENESIS

Based on PCR primer design, any type of mutation, such as deletions, insertions, or substitutions, can be introduced into your genes in a single or multiple sites. Any desired changes in amino acids, or deletions and insertions of restriction sites can be made into the genes of your interest.

 Gene Mutagenesis Service Description:

- Design and synthesis of mutagenesis primers
- PCR amplification
- Confirmation of desired mutations by DNA sequencing
- Confirmation of undesired mutations by DNA sequencing
- Subcloning modified gene into fresh vector not subjected to
  the mutagenesis procedure or subcloning into another vector   (Optional)
- Verification of vector sequence after mutagenesis by DNA
  sequencing (Optional)
- Delivery of purified plasmid DNA construct and its glycerol
  stock

View Report Example


 RANDOM MUTAGENESIS

We create a random mutagenesis library of any desired complexity to any gene of your interest.

 Service Description:

- Cloning of the randomized DNA into any vector
- Cloning into expression vectors
- Confirmation of desired mutation by DNA sequencing
- Selection of mutation frequencies depending on the   application of the study