Full Sequencing Analysis
Double-stranded Sequencing
ACGT, Inc. offers a Full Sequence Analysis service to confirm the sequences of
a clone or detection of mutations or deletions. Our service is comprehensive and
results in double-strand sequence data of publication quality.
Full Sequencing Analysis Service Description:
- DNA sequencing by the primer walking method with multiple overlap
- Design and synthesis of internal primers specific to your template DNA at every 600-650 bases
- A thorough examination of the chromatograms and data editing
- Sequence data assembly and alignment
- Checking of every base for discrepancies
- Determination of consensus sequence
Single-stranded Sequencing
Our single-stranded sequencing consists of assembly of primer extension sequence
data and resolution of sequence ambiguities.
Service Description:
- Design and synthesis of internal primers specific to your template DNA at every 600-650 bases in single strand
- A thorough examination of the chromatograms and data editing
- Overlapping sequence data assembly and alignment
- Checking of every base for discrepancies in
overlapping
regions - Determination of consensus sequence in overlapping regions
TRANSPOSON INSERTION SEQUENCING
For extra large size DNA or completely unknown DNA, ACGT, Inc. offers the
Transposon Insertion sequencing method.
Service Description:
- Insertion of transposon in every 550-600 bases to ensure multiple redundancies
- A thorough examination of the chromatograms and data editing
- Sequence data assembly and alignment
- Gap closure by primer walking method
- Checking of every base for errors and discrepancies
- Determination of consensus sequence
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