Full Sequencing Analysis
DOUBLE-STRANDED SEQUENCING
ACGT, Inc. offers a Full Sequence Analysis service to confirm the sequences of
a clone or detection of mutations or deletions. Our service is comprehensive and
results in double-strand sequence data of publication quality.
Full Sequencing Analysis Service Description:
- DNA sequencing by the primer walking method with multiple
overlap
- Design and synthesis of internal primers specific to your
template DNA at every 600-650 bases
- A thorough examination of the chromatograms and data
editing
- Sequence data assembly and alignment
- Checking of every base for discrepancies
- Determination of consensus sequence
View Report Examples
SINGLE-STRANDED SEQUENCING
Our single-stranded sequencing consists of assembly of primer extension sequence
data and resolution of sequence ambiguities.
Service Description:
- Design and synthesis of internal primers specific to your template DNA at every
600-650 bases in single strand
- A thorough examination of the chromatograms and data editing
- Overlapping sequence data assembly and alignment
- Checking of every base for discrepancies in
overlapping
regions
- Determination of
consensus sequence in overlapping regions
View Report Examples
TRANSPOSON INSERTION SEQUENCING
For extra large size DNA or completely unknown DNA, ACGT, Inc. offers the
Transposon Insertion sequencing method.
Service Description:
- Insertion of transposon in every 550-600 bases to ensure multiple redundancies
- A thorough examination of the chromatograms and data editing
- Sequence data assembly and alignment
- Gap closure by primer walking method
- Checking of every base for errors and discrepancies
- Determination of consensus sequence
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