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dna sequencing

Frequently asked questions

Questions Before Submitting Samples:


Q1. How can I place an order?
Q2. How can I open an online account?
Q3. I haven't sequenced for a while, what do we need to do to bring my records up to date and start sequencing again?
Q4. What is the average number of bases generated by single pass primer extension?
Q5. My DNA is much larger than 950 bases. How can I determine the sequence for the whole thing?
Q6. I have a cloned fragment of more than 50 kb. How can I determine the sequence for the whole thing?
Q7. I am looking for mutations in a known sequence. How can you help me to speed up the procedure?
Q8. I have a clone that has absolutely no sequence or vector information. How can you help me?
Q9. Can you prepare DNA samples for single/double strand sequencing?
Q10. What is the accuracy for single pass primer extension?
Q11. What is the accuracy for double strand full sequencing?
Q12. What standard primers do you provide?
Q13. Do you check all samples received on agarose gel before sequencing?
Q14. Can ACGT, Inc. sequence difficult templates, e.g. G-C rich samples?
Q15. Can I use PCR primers to sequence PCR reactions?
Q16. What kinds of tubes should I use?
Q17. How do I name my samples?
Q18. How do I label my samples?
Q19. Can I put the template and primer in the same tube?
Q20. If I don’t have an internal primer, do you synthesize custom primers?
Q21. Can I ship out the samples on Friday?
Q22. What is ACGT, Inc.’s re-sequencing policy?
Q23. What concentration or volume is required for each DNA sample?
Q24. How do I send samples?



GLP Compliant Facility • 35 Waltz Drive, Wheeling, IL 60090 • P: 800.557.ACGT (2248) • F: 847.520.9163 • Email: dnaseq@acgtinc.com