DNA and RNA Extraction
ACGT, Inc. offers DNA and RNA extraction services for small and large-scale projects. We are experienced with extracting and purifying nuclei acids from various sources including tissue (fresh and preserved), blood, saliva, other body fluids, cell cultures, plants, and forensics samples.
Genomic DNA Extraction
Intact, high-quality genomic DNA (gDNA) is routinely extracted from blood, saliva, buccal swabs, soft tissues, or plants. We also perform total nuclei acid extractions where gDNA, plasmid DNA and RNA are concomitantly isolated from the same sample such as bacteria. For DNA extraction from plants, the Automated Sample Grinding System (AutoGen) is used to obtain consistent results. Extraction of gDNA from rodent tails or ears is part of our transgenic genotyping service.
DNA Extraction Service Description:
- DNA extraction
- Quantitative analysis by UV spectroscopy and PicoGreen
- Qualitative analysis by UV spectroscopy and agarose gel
- Sample concentration adjustment per customer specifications
- Aliquot of sample into tubes or plates, and shipping to any destination
Plasmid DNA Isolation
High quality plasmid DNA from the Mini to Giga scale is available for many applications including DNA sequencing, transfections, and DNA-mediated antibody production. Resin based methods are used to prepare highly pure plasmid DNA preparations with an option of making these endotoxin-free. Comparable services are available for BAC, lambda, and cosmid DNA. Expected yields of plasmid DNA from bacteria is given below.
For high copy plasmid DNA:
| Types | Expected DNA Yield |
| Mini | 2 - 20 µg |
| Midi | 20 - 100 µg |
| Maxi | 100-1,000 µg |
| Mega | 1-5 mg |
| Giga | 5-20 mg |
For low copy plasmid DNA:
| Types | Expected DNA Yield |
| Mini | 0.1 - 10 µg |
| Midi | 10 - 50 µg |
| Maxi | 50 - 500 µg |
| Mega | 0.5 - 2.5 mg |
| Giga | 2.5 -10 mg |
RNA Extraction
Extraction of highly pure and intact RNA (total, mRNA, sRNA and miRNA) from a variety of samples including soft tissues (fresh and preserved), blood, saliva, cell cultures, plants, and forensics samples is available whether it from a few samples to large-scale project. Both liquid and resin based purification systems are used to extract the RNA species of interest. Concomitant extraction of genomic DNA and plasmid DNA is possible. The purified RNA is suitable for many downstream applications including cDNA synthesis including library construction, Northern blots and qPCR.
Service Description:
- RNA extraction by liquid or resin based methods
- Quantitative analysis by UV spectroscopy and RiboGreen
- Qualitative analysis by UV spectroscopy and agarose gel
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