cDNA Services    

Our cDNA services for cDNA are comprehensive and customized to meet your applications. Service ranges from full-length cDNA cloning to cDNA library screening.

 cDNA CLONING

- Extraction of RNA from a variety of samples
- Full length and partial cDNA cloning
- Cloning via PCR, RT-PCR, 5' and 3' RACE
- Cloning into any desired vector
- Cloning open reading frame into any expression
  vector
- Customize protein expression by inclusion of epitope   tags or fusion constructs such as green fluorescent
  protein at either end of protein

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 cDNA LIBRARY CONSTRUCTION

- Extraction of RNA from a variety of samples
- Generation of phage- or plasmid-based cDNA   libraries from size-selected cDNA
- Directional cloning of cDNAs into any vector
- Production of a library with at least 10 cfu
- Library amplification to generate higher titer   library
- Library delivered on plates, filters or glycerol   stock
- Verification of library quality by randomly   picking clones and determining insert sizes by   restriction digest
- Verification of library complexity by DNA   sequencing the inserts from a subset of   randomly picked clones (Optional)