cDNA Library Construction

cDNA Library Construction Service Description:

  • Extraction of RNA from a variety of samples
  • Generation of phage- or plasmid-based cDNA libraries from size-selected cDNA
  • Directional cloning of cDNAs into any vector
  • Production of a library with at least 10 cfu
  • Library amplification to generate higher titer library
  • Library delivered on plates, filters or glycerol stock
  • Verification of library quality by randomly picking clones and determining insert sizes by restriction digest
  • Verification of library complexity by DNA sequencing of the inserts from a subset of randomly picked clones (Optional)